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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in lots of tumors, which leads to chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant part in marketing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two more splice variants ended up reported but comprehensive nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.
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This guideline outlines further attainable optimization of cellular and immunoassay parameters to ensure the very best outcomes are...
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This information outlines even more doable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies bound to the blocking peptide now not bind to the epitope around the target protein. By evaluating the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Info Cyclin E belongs towards the very conserved cyclin household, whose associates are characterized by a remarkable periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Unique cyclins show distinct expression and degradation patterns which lead towards the temporal coordination of each and every mitotic occasion. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S phase boundary which is degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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